Genetically Engineered Mice by Pronuclear DNA Microinjection
نویسندگان
چکیده
منابع مشابه
Review Production of functional transgenic mice by DNA pronuclear microinjection
Successful experiments involving the production of transgenic mice by pronuclear microinjection are currently limited by low efficiency of random transgene integration into the mouse genome. Furthermore, not all transgenic mice express integrated transgenes, or in other words are effective as functional transgenic mice expressing the desired product of the transgene, thus allowing accomplishmen...
متن کاملProduction of functional transgenic mice by DNA pronuclear microinjection.
Successful experiments involving the production of transgenic mice by pronuclear microinjection are currently limited by low efficiency of random transgene integration into the mouse genome. Furthermore, not all transgenic mice express integrated transgenes, or in other words are effective as functional transgenic mice expressing the desired product of the transgene, thus allowing accomplishmen...
متن کاملPronuclear Microinjection of Rabbit Fertilized Eggs
Although, the first transgenic animal was produced more than 30 years ago, there are still many factors limiting efficiency of transgenesis. One of them is the low rate of transgene incorporation into the genome of microinjected eggs. This mini-review summarizes recent research methods based on pronuclear microinjection of rabbit fertilized eggs in order to reach higher efficiency of transgene ...
متن کاملA method for the generation of YAC transgenic mice by pronuclear microinjection.
Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has become an important goal, since it offers the potential to study the control of large and complex transcription units and identify genes by complementation. Microinjection into the nucleus is the most ...
متن کاملGerm line transformation of mammals by pronuclear microinjection.
The most popular approach for generating transgenic mammals is the direct injection of transgenes into one pronucleus of a fertilized oocyte. In the past 15 years microinjection has been successfully applied in laboratory as well as in farm animals. The frequency of transgenic founders, although highly different between the species, is efficient enough to render this technique applicable to a w...
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ژورنال
عنوان ژورنال: Current Protocols in Mouse Biology
سال: 2012
ISSN: 2161-2617
DOI: 10.1002/9780470942390.mo110168